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23s/16s rRNA ratio

23s/16s rRNA ratio - Protocols and Methods Forum

23s/16s rRNA ratio - Post Any Protocol, Method, Technique, Procedure or Tips / Troubleshooting for any Molecular Biology Technique.

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Old 09-02-2007, 09:09 PM
yotsawat pomyen
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Default 23s/16s rRNA ratio

Hi all,

I have to extract total RNA from E.coli for microarray
experiment. I analyzed my RNA using 2100 Bioanalyzer
and it provides information about rRNA ratio. I did
some search and found that mostly 23s/16s rRNA ratio
for good RNA quality is about 2:1. My results from
Bioanalyzer are vary from 1.8 to 2.6.
My questions are:
1. What rRNA ratio tell me about my RNA?
2. Why they said that the 23s/16s ratio of 2:1 is
good? Why 2:1?
3. Is my RNA in good shape judging from rRNA ratio
that I got?

Thank you

Yotsawat Pomyen

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Old 09-04-2007, 09:56 AM
Posts: n/a
Default 23s/16s rRNA ratio

"yotsawat pomyen" <[Only registered users see links. ]> wrote in message
news:[Only registered users see links. ]. net...
In general the 23S rRNA is more labile than the 16S. So the ratio will
decrease as the 23S degrades. The lower the ratio the more degradation has

There are more nucleotides per molecule to bind the dye used in the assay so
the 23S peak should be larger given a 1:1 molecular ratio of rRNA subunits.
I guess the exact figure is dependant on the organism.

Yes. Other things to look for are 23/16S peaks of about the same height and
a relatively flat baseline. Agilent now sell software which gives an RNA
Integrity Number but I am not sure how well validated it is for prokaryotic



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23s or 16s , ratio , rrna

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