Go Back   Science Forums Biology Forum Molecular Biology Forum Physics Chemistry Forum > Molecular Research Topics Forum > Protocols and Methods Forum
Register Search Today's Posts Mark Forums Read

Protocols and Methods Forum Post Any Protocol, Method, Technique, Procedure or Tips / Troubleshooting for any Molecular Biology Technique.


23s/16s rRNA ratio

23s/16s rRNA ratio - Protocols and Methods Forum

23s/16s rRNA ratio - Post Any Protocol, Method, Technique, Procedure or Tips / Troubleshooting for any Molecular Biology Technique.


Reply
 
LinkBack Thread Tools Display Modes
  #1  
Old 09-02-2007, 09:09 PM
yotsawat pomyen
Guest
 
Posts: n/a
Default 23s/16s rRNA ratio



Hi all,

I have to extract total RNA from E.coli for microarray
experiment. I analyzed my RNA using 2100 Bioanalyzer
and it provides information about rRNA ratio. I did
some search and found that mostly 23s/16s rRNA ratio
for good RNA quality is about 2:1. My results from
Bioanalyzer are vary from 1.8 to 2.6.
My questions are:
1. What rRNA ratio tell me about my RNA?
2. Why they said that the 23s/16s ratio of 2:1 is
good? Why 2:1?
3. Is my RNA in good shape judging from rRNA ratio
that I got?

Thank you

Yotsawat Pomyen




__________________________________________________ __________________________________
Be a better Heartthrob. Get better relationship answers from someone who knows. Yahoo! Answers - Check it out.
[Only registered users see links. ]

Reply With Quote
  #2  
Old 09-04-2007, 09:56 AM
IanMc
Guest
 
Posts: n/a
Default 23s/16s rRNA ratio

"yotsawat pomyen" <[Only registered users see links. ]> wrote in message
news:[Only registered users see links. ]. net...
In general the 23S rRNA is more labile than the 16S. So the ratio will
decrease as the 23S degrades. The lower the ratio the more degradation has
ocurred.


There are more nucleotides per molecule to bind the dye used in the assay so
the 23S peak should be larger given a 1:1 molecular ratio of rRNA subunits.
I guess the exact figure is dependant on the organism.


Yes. Other things to look for are 23/16S peaks of about the same height and
a relatively flat baseline. Agilent now sell software which gives an RNA
Integrity Number but I am not sure how well validated it is for prokaryotic
organisms.

Regards,

IanMc



Reply With Quote
Reply

Tags
23s or 16s , ratio , rrna


Thread Tools
Display Modes

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off
Trackbacks are On
Pingbacks are On
Refbacks are On

Forum Jump

Similar Threads
Thread Thread Starter Forum Replies Last Post
Ligation: j/i ratio Ferdinando Pucci Protocols and Methods Forum 0 07-25-2004 04:28 PM
Ratio of light-->matter between galaxys, question simplesam Physics Forum 2 04-07-2004 09:58 PM
New Saccharomyces Sequences 02/25/04 SGD Sequences Yeast Forum 0 02-27-2004 04:23 AM
New Saccharomyces Sequences 02/18/04 SGD Sequences Yeast Forum 0 02-18-2004 08:06 PM
23S/16S ratios in RNA preps dependent on isolation method? OBF Protocols and Methods Forum 6 01-27-2004 01:14 AM


All times are GMT. The time now is 09:59 AM.


Powered by vBulletin® Version 3.8.4
Copyright ©2000 - 2014, Jelsoft Enterprises Ltd.
Copyright 2005 - 2012 Molecular Station | All Rights Reserved
Page generated in 0.11917 seconds with 16 queries