Go Back   Science Forums Biology Forum Molecular Biology Forum Physics Chemistry Forum > Molecular Research Topics Forum > Protocols and Methods Forum
Register Search Today's Posts Mark Forums Read

Protocols and Methods Forum Post Any Protocol, Method, Technique, Procedure or Tips / Troubleshooting for any Molecular Biology Technique.


Diferential primer design

Diferential primer design - Protocols and Methods Forum

Diferential primer design - Post Any Protocol, Method, Technique, Procedure or Tips / Troubleshooting for any Molecular Biology Technique.


Reply
 
LinkBack Thread Tools Display Modes
  #1  
Old 08-31-2007, 11:02 AM
Yoram Gerchman
Guest
 
Posts: n/a
Default Diferential primer design



Greetings all
I am trying to design primer to differentiate via real-time PCR, the expression
of 4 homologous genes. Does anyone know of a software or webpage that will
enable me to define the primer search so that the primer will bind to sequence
1 but not to sequences 2,3 and 4?
Many thanks
Yoram


------------------------------------------------------------------------
This message was sent using IMP, the Webmail Program of Haifa University

Reply With Quote
  #2  
Old 09-01-2007, 01:56 AM
Tom Knight
Guest
 
Posts: n/a
Default Diferential primer design

Yoram Gerchman <[Only registered users see links. ].il> writes:


Primers fail to prime if they mismatch at the 3' end, even by a single
base. You can design the primers you want just by a clustalW
alignment of the homologous genes and searches for locations with
mismatches.

Reply With Quote
Reply

Tags
design , diferential , primer


Thread Tools
Display Modes

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off
Trackbacks are On
Pingbacks are On
Refbacks are On

Forum Jump

Similar Threads
Thread Thread Starter Forum Replies Last Post
Can anyone help me to correct my primer design? confusing PCR - Polymerase Chain Reaction Forum 2 01-12-2010 10:12 PM
Can anyone help me to correct my primer design? confusing Bioinformatics 0 12-12-2009 12:21 PM
Bad primer design ? How can I know ? surt Bioinformatics 4 10-07-2009 07:24 AM
How to design primer? af malik PCR - Polymerase Chain Reaction Forum 3 01-29-2009 11:11 AM
Diferential primer design Glenn Dunshea Protocols and Methods Forum 0 08-31-2007 11:04 PM


All times are GMT. The time now is 04:56 PM.


Powered by vBulletin® Version 3.8.4
Copyright ©2000 - 2014, Jelsoft Enterprises Ltd.
Copyright 2005 - 2012 Molecular Station | All Rights Reserved
Page generated in 0.11526 seconds with 16 queries