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transgene insertion and repetitive sequences

transgene insertion and repetitive sequences - Protocols and Methods Forum

transgene insertion and repetitive sequences - Post Any Protocol, Method, Technique, Procedure or Tips / Troubleshooting for any Molecular Biology Technique.


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Old 08-16-2007, 05:58 PM
Ed Siefker
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Default transgene insertion and repetitive sequences



Tom Anderson wrote:

I'm not sure I understand this. We do know that the transgene is in
band 3A3. If I get a BAC from this region, how would I use it as a
probe? It's kind of big for that isn't it?



Hmmm. Those are good ideas, I hadn't thought about gel purifying the
digest. I should be able to get rid of the endogenous X chromosome
sequence that way too.



Hey neat, I didn't know they could do that.


Yeah, I'll have to ask the people who did our FISH if they can get us
any finer mapping. Thanks a lot for the ideas.
-Ed Siefker

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Thread Thread Starter Forum Replies Last Post
transgene insertion and repetitive sequences Tom Anderson Protocols and Methods Forum 0 08-17-2007 02:30 PM
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transgene insertion and repetitive sequences Ed Siefker Protocols and Methods Forum 0 08-14-2007 07:04 PM


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