transgene insertion and repetitive sequences

Tom Anderson wrote:

I'm not sure I understand this. We do know that the transgene is in
band 3A3. If I get a BAC from this region, how would I use it as a
probe? It's kind of big for that isn't it?



Hmmm. Those are good ideas, I hadn't thought about gel purifying the
digest. I should be able to get rid of the endogenous X chromosome
sequence that way too.



Hey neat, I didn't know they could do that.


Yeah, I'll have to ask the people who did our FISH if they can get us
any finer mapping. Thanks a lot for the ideas.
-Ed Siefker