|Register||Search||Today's Posts||Mark Forums Read|
|Protocols and Methods Forum Post Any Protocol, Method, Technique, Procedure or Tips / Troubleshooting for any Molecular Biology Technique.|
| ||LinkBack||Thread Tools||Display Modes|
transgene insertion and repetitive sequences
Tom Anderson wrote:
I'm not sure I understand this. We do know that the transgene is in
band 3A3. If I get a BAC from this region, how would I use it as a
probe? It's kind of big for that isn't it?
Hmmm. Those are good ideas, I hadn't thought about gel purifying the
digest. I should be able to get rid of the endogenous X chromosome
sequence that way too.
Hey neat, I didn't know they could do that.
Yeah, I'll have to ask the people who did our FISH if they can get us
any finer mapping. Thanks a lot for the ideas.
|insertion , repetitive , sequences , transgene|
|Thread||Thread Starter||Forum||Replies||Last Post|
|transgene insertion and repetitive sequences||Tom Anderson||Protocols and Methods Forum||0||08-17-2007 02:30 PM|
|transgene insertion and repetitive sequences||Tom Anderson||Protocols and Methods Forum||0||08-15-2007 12:41 PM|
|transgene insertion and repetitive sequences||Ed Siefker||Protocols and Methods Forum||0||08-14-2007 07:04 PM|