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inert dye for real-time pcr

inert dye for real-time pcr - Protocols and Methods Forum

inert dye for real-time pcr - Post Any Protocol, Method, Technique, Procedure or Tips / Troubleshooting for any Molecular Biology Technique.


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Old 08-02-2007, 02:38 PM
Tim Fitzwater
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Default inert dye for real-time pcr



"I'm looking for an inert dye to add to my colorless real-time pcr
mixes to be able to check the performance of a pipetting robot."



There are published reports of two different red dyes used for this
purpose.



1) 0.01% Phenol red (R. J. Klebe, S. A. Rodriguez, M. L. VerBeek and T.
A.

Giambernardi, 1999. Simple method for "hot-start" RT-PCR.

BioTechn. 27(6):1108-1110.)



2) Carl Wittwer's laboratory uses 0.1 mM Cresol Red for the same purpose


(The Rapid Cyclist, Spring, 1994, page 9).



Tim

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Old 08-02-2007, 05:25 PM
peter
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Default inert dye for real-time pcr

On Aug 2, 10:38 am, "Tim Fitzwater" <[Only registered users see links. ]> wrote:

Since PCR mix is H2O based you can try any number of food color dyes,
provided that they wont interfere with the emission/excitation spectra
of your real-time detection system.
Peter

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