inert dye for real-time pcr

I'm looking for an inert dye to add to my colorless real-time pcr
mixes to be able to check the performance of a pipetting robot. Of
course there are commercial solutions like Thermo's Absolute Blue but
these are not an option. Years ago in this group tatrazine was
mentioned as a possible dye. Does anyone know more ????

Thanks to you all

Emilio

jcww99 <[Only registered users see links. ]> writes:

Cresol Red is another option. Just a quick warning for a problem we
encountered, which is that some of these dyes fluoresce. This is not
normally a problem, but can completely trash a sequencing reaction if
dye manages to get into the DNA template. There may be issues with
real time PCR detection as well.