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inert dye for real-time pcr

inert dye for real-time pcr - Protocols and Methods Forum

inert dye for real-time pcr - Post Any Protocol, Method, Technique, Procedure or Tips / Troubleshooting for any Molecular Biology Technique.


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Old 08-01-2007, 09:47 PM
jcww99
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Default inert dye for real-time pcr



I'm looking for an inert dye to add to my colorless real-time pcr
mixes to be able to check the performance of a pipetting robot. Of
course there are commercial solutions like Thermo's Absolute Blue but
these are not an option. Years ago in this group tatrazine was
mentioned as a possible dye. Does anyone know more ????

Thanks to you all

Emilio
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Old 08-02-2007, 03:19 PM
Tom Knight
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Default inert dye for real-time pcr

jcww99 <[Only registered users see links. ]> writes:

Cresol Red is another option. Just a quick warning for a problem we
encountered, which is that some of these dyes fluoresce. This is not
normally a problem, but can completely trash a sequencing reaction if
dye manages to get into the DNA template. There may be issues with
real time PCR detection as well.


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