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HPLC-ECD of Oxytocin Using an Ab Column

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Old 06-21-2007, 05:29 PM
tocopherol
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Default HPLC-ECD of Oxytocin Using an Ab Column



Isocratic High Performance Liquid Chromatography - Coulometric
Electrochemical Detection of Oxytocin in Biological Samples Using C18
Solid Phase Extraction and Polyclonal Antibody-Based Immunoaffinity
Column Purification

by Dr. Mark A. Kukucka and Dr. Hara P. Misra

Department of Biomedical Sciences
Virginia-Maryland Regional College of Veterinary Medicine
Virginia Polytechnic Institute & State University
Blacksburg, Virginia 24061-0442

Published in: Journal of Chromatography - Biomedical Applications


ABSTRACT: A rapid, sensitive and very specific high-performance liquid
chromatographic (HPLC) method is described for the reliable and
selective quantitation of oxytocin using culture media supernatants.
The isocractic procedure employs solid phase extraction, antibody-
based immunoaffinity purification and dual
channel coulometric electrochemical detection (ECD). The lower limit
of detection for this octapeptide was 40 picograms (40 fmol) on
column. Due to its relative simplicity, specificity and precision, the
HPLC-ECD of oxytocin is an accurate and attractive alternative to many
existing quantitative methods.


Running Title: HPLC-ECD of Oxytocin Using an Ab Column

Key Words: Oxytocin, HPLC, ECD, SPE, Antibody column


Kukucka, Mark A. and Hara P. Misra. 1994. Determination of oxytocin
in biological samples by isocratic high-performance liquid
chromatography with coulometric detection using C18 solid-phase
extraction and polyclonal antibody-based immunoaffinity column
purification. J Chromatogr B Biomed Appl 653(2):139-145.

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