Mitochondrial marker protein?

Hi all,

any ideas for a good mitochondrial marker protein for human skeletal
muscle which is easily to detect in westerns? a molecular weight above
25kd would be fine.

Many thanks for your suggestions!

Wo

On Wed, 20 Jun 2007, WS wrote:


Prohibitin is a popular choice for this.

A colleague is working on the growth and replication of mitochondria, and
he's spent a lot of time thinking about how to quantify them. A
fundamental problem here is that the protein composition of mitochondria
changes according to signals and growth conditions, which means it's not
clear what 'amount' means - if you have the same amount of F0 ATPase but
twice as much of some matix enzyme, say SDH, do you have the same amount
of mitochondrion, or twice as much? Does it matter if some given mass of
proteins is divided between N mitochondria or 2N? I think he decided that
the gold standard was the number of copies of the mtDNA, but this is a
nightmare to measure; for most of his work, he's stained with MitoTracker
and measured the total volume of mitochondria in the cell. I'll ask him if
he has any suggestions about markers for blots, though.

You might also like to have a look at:

[Only registered users see links. ]

tom

--
Tom Anderson, MRC Laboratory for Molecular Cell Biology, UCL, London WC1E 6BT
(t) +44 (20) 76797264 (f) +44 (20) 76797805 (e) [Only registered users see links. ]

[binumerm1]

Thanks Tom for that! I had a quick question.. Currently I am trying to quantify mitochondrial proteins using scintillation counts. The biggest challenge I am facing is repeatability of the results plus protein estimation using assays such as Bradford. Do you have any suggestion on what assay is preferred to measure mitochondrial proteins? Since my goal is to look at the effects of translation rate I usually divide the CPM (from the scintillation) by the protein concentration (from the bradford assay).
Thanks,
Anwar