Go Back   Science Forums Biology Forum Molecular Biology Forum Physics Chemistry Forum > Molecular Research Topics Forum > Protocols and Methods Forum
Register Search Today's Posts Mark Forums Read

Protocols and Methods Forum Post Any Protocol, Method, Technique, Procedure or Tips / Troubleshooting for any Molecular Biology Technique.


Mitochondrial marker protein?

Mitochondrial marker protein? - Protocols and Methods Forum

Mitochondrial marker protein? - Post Any Protocol, Method, Technique, Procedure or Tips / Troubleshooting for any Molecular Biology Technique.


Reply
 
LinkBack Thread Tools Display Modes
  #1  
Old 06-20-2007, 04:33 PM
WS
Guest
 
Posts: n/a
Default Mitochondrial marker protein?



Hi all,

any ideas for a good mitochondrial marker protein for human skeletal
muscle which is easily to detect in westerns? a molecular weight above
25kd would be fine.

Many thanks for your suggestions!

Wo

Reply With Quote
  #2  
Old 06-21-2007, 12:58 PM
Tom Anderson
Guest
 
Posts: n/a
Default Mitochondrial marker protein?

On Wed, 20 Jun 2007, WS wrote:


Prohibitin is a popular choice for this.

A colleague is working on the growth and replication of mitochondria, and
he's spent a lot of time thinking about how to quantify them. A
fundamental problem here is that the protein composition of mitochondria
changes according to signals and growth conditions, which means it's not
clear what 'amount' means - if you have the same amount of F0 ATPase but
twice as much of some matix enzyme, say SDH, do you have the same amount
of mitochondrion, or twice as much? Does it matter if some given mass of
proteins is divided between N mitochondria or 2N? I think he decided that
the gold standard was the number of copies of the mtDNA, but this is a
nightmare to measure; for most of his work, he's stained with MitoTracker
and measured the total volume of mitochondria in the cell. I'll ask him if
he has any suggestions about markers for blots, though.

You might also like to have a look at:

[Only registered users see links. ]

tom

--
Tom Anderson, MRC Laboratory for Molecular Cell Biology, UCL, London WC1E 6BT
(t) +44 (20) 76797264 (f) +44 (20) 76797805 (e) [Only registered users see links. ]
Reply With Quote
  #3  
Old 01-15-2009, 05:43 PM
Pipette Filler
Points: 813, Level: 16 Points: 813, Level: 16 Points: 813, Level: 16
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: Dec 2007
Posts: 1
Thanks: 0
Thanked 0 Times in 0 Posts
Default Re: Mitochondrial marker protein?

Thanks Tom for that! I had a quick question.. Currently I am trying to quantify mitochondrial proteins using scintillation counts. The biggest challenge I am facing is repeatability of the results plus protein estimation using assays such as Bradford. Do you have any suggestion on what assay is preferred to measure mitochondrial proteins? Since my goal is to look at the effects of translation rate I usually divide the CPM (from the scintillation) by the protein concentration (from the bradford assay).
Thanks,
Anwar
Reply With Quote
Reply

Tags
marker , mitochondrial , protein


Thread Tools
Display Modes

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off
Trackbacks are On
Pingbacks are On
Refbacks are On

Forum Jump

Similar Threads
Thread Thread Starter Forum Replies Last Post
Human Cytome Project - Update 24 Jan. 2005 Peter Van Osta Cell Biology and Cell Culture 1 08-01-2010 02:18 PM
Human Cytome Project - an idea - Update 19 April 2005 Peter Van Osta Cell Biology and Cell Culture 1 06-01-2009 02:17 PM
Human Cytome Project - Update 6 Jan. 2005 Peter Van Osta Cell Biology and Cell Culture 0 01-06-2005 11:18 AM
New Saccharomyces Sequences 11/27/04 Mike Cherry Yeast Forum 0 11-29-2004 12:39 AM
New Saccharomyces Sequences 05/19/04 SGD Sequences Yeast Forum 0 05-23-2004 04:06 PM


All times are GMT. The time now is 03:27 AM.


Powered by vBulletin® Version 3.8.4
Copyright ©2000 - 2014, Jelsoft Enterprises Ltd.
Copyright 2005 - 2012 Molecular Station | All Rights Reserved
Page generated in 0.17816 seconds with 16 queries