Go Back   Science Forums Biology Forum Molecular Biology Forum Physics Chemistry Forum > Molecular Research Topics Forum > Protocols and Methods Forum
Register Search Today's Posts Mark Forums Read

Protocols and Methods Forum Post Any Protocol, Method, Technique, Procedure or Tips / Troubleshooting for any Molecular Biology Technique.


Strong contamination with ethidium bromide

Strong contamination with ethidium bromide - Protocols and Methods Forum

Strong contamination with ethidium bromide - Post Any Protocol, Method, Technique, Procedure or Tips / Troubleshooting for any Molecular Biology Technique.


Reply
 
LinkBack Thread Tools Display Modes
  #1  
Old 06-05-2007, 04:33 PM
Jayakumar, R
Guest
 
Posts: n/a
Default Strong contamination with ethidium bromide




You could probably try amputating your finger tips or hope that
localized tumors develop for the sake of science. Anyway, Ethidium
bromide is a suspected mutagen (being DNA intercalating), there has been
no report of anybody actually having developed tumors or cancer because
of it at the levels we use in the lab (maybe in mice). If you develop
one, you could probably study it and publish a paper in nature. There
is always a good side to everything.
On the more serious side, exercise great caution while handling it.
Wear gloves (nitrile is very good) and decontaminate the area of
spillage as per your instituttes guidelines (I wipe it down with butanol
several times and then use RED-Z to remove the washates (prevented if
you use plastic backed desk protectors). Dispose solid and liquid wastes
carefully as per environmental guidelines. Even if you are careless,
don't let your carelessness affect others in the lab and your family. So
wear protective gear and report spillages to everyone in the lab.

Jay




-----Original Message-----
From: [Only registered users see links. ]
[mailto:[Only registered users see links. ].indiana.edu] On Behalf Of peter
Sent: Tuesday, June 05, 2007 9:08 AM
To: [Only registered users see links. ]
Subject: Re: Strong contamination with ethidium bromide

On Jun 4, 6:05 pm, Undergrad <[Only registered users see links. ]> wrote:
a graveyard ?
context:[Only registered users see links. ]
-tf38...

Don't worry you will be fine...EtBr has been investigated as a drug
against cancer in the past, not very effective ....
Peter

_______________________________________________
Methods mailing list
[Only registered users see links. ]
[Only registered users see links. ]


This email message may contain legally privileged and/or confidential information. If you are not the intended recipient(s), or the employee or agent responsible for the delivery of this message to the intended recipient(s), you are hereby notified that any disclosure, copying, distribution, or use of this email message is prohibited. If you have received this message in error, please notify the sender immediately by e-mail and delete this email message from your computer. Thank you.

Reply With Quote
Reply

Tags
bromide , contamination , ethidium , strong


Thread Tools
Display Modes

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off
Trackbacks are On
Pingbacks are On
Refbacks are On

Forum Jump

Similar Threads
Thread Thread Starter Forum Replies Last Post
ethidium bromide contamination on clothes Jayakumar, R Protocols and Methods Forum 12 07-14-2010 05:01 PM
RE to Jim 3975 - Strong contamination with ethidium bromide Prof. Piero Sestili Protocols and Methods Forum 17 06-14-2007 04:02 PM
Strong contamination with ethidium bromide Undergrad Protocols and Methods Forum 6 06-14-2007 03:12 PM
RE to Jim 3975 - Strong contamination with ethidium bromide Jayakumar, R Protocols and Methods Forum 0 06-11-2007 04:42 PM
Nobel Prize for David Thomson?! caltechdude Physics Forum 428 01-21-2005 10:39 PM


All times are GMT. The time now is 06:56 AM.


Powered by vBulletin® Version 3.8.4
Copyright ©2000 - 2014, Jelsoft Enterprises Ltd.
Copyright 2005 - 2012 Molecular Station | All Rights Reserved
Page generated in 0.12110 seconds with 16 queries