Since 3 weeks I am using Ethidium bromide without taking much more care of
it. Everyday I used it and I notice that few drops of the stock solution
were throw on my finger each time I opened the tube. I never washed
afterwards. I estimated that I have done it at least 10 times. I just read
how toxic is ethidium bromide and now I am very scared about had
contaminated myself. Some people says that ethidium bromide fear is paranoia
but if not what should I do? Book a place in a graveyard ?
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In article <[Only registered users see links. ]>, Kyle Legate <[Only registered users see links. ]> wrote:
[I realize it's a joke but anyway]
EthBr is clearly overrated but AA's reputation is well-deserved.
Chronic consumption of AA (apparently, above some threshold)
can really can screw you up. In some cases, paralysis occurs and
it is irreversible.
That said, I never wear gloves while pouring gels. Gloves are
abused in today's labs to the point of them being bigger problem
than the problems they are supposed to protect from.
At least one should think about were to use them. Diaminobenzidine for
example is a very fine powder, if you handle an open bottle with gloves
the static electricity will blow it right into your face. But your hands,
of course, are protected.
Removing Eppendorf vials with radioactive samples from a waterbath is
another example. The things are so slippery that you easily splash the
sample across the entire room. I do use gloves when aliquoting radioactive
stocks, but for the few nCi in a sample gloves are indeed more of a
problem than a solution.
To the OP: Before you start working with a substance you should read up on
its properties. Bottle labels and catalogues contain the most important
info like R/S statements. In addition, there are material safety data
sheets (MSDS) available via suppliers web sites. Your lab may also have
printed copies for the chemicals they use (required in some
jurisdictions). There should also be reasonable supervision and training
of new staff members, letting them handle dangerous chemicals without
training is simply criminal.