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Strong contamination with ethidium bromide

Strong contamination with ethidium bromide - Protocols and Methods Forum

Strong contamination with ethidium bromide - Post Any Protocol, Method, Technique, Procedure or Tips / Troubleshooting for any Molecular Biology Technique.


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  #1  
Old 06-04-2007, 10:05 PM
Undergrad
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Default Strong contamination with ethidium bromide




Since 3 weeks I am using Ethidium bromide without taking much more care of
it. Everyday I used it and I notice that few drops of the stock solution
were throw on my finger each time I opened the tube. I never washed
afterwards. I estimated that I have done it at least 10 times. I just read
how toxic is ethidium bromide and now I am very scared about had
contaminated myself. Some people says that ethidium bromide fear is paranoia
but if not what should I do? Book a place in a graveyard ?

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  #2  
Old 06-05-2007, 08:58 AM
Peter Ellis
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Default Strong contamination with ethidium bromide

Undergrad wrote:

Start taking more care.

Peter


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  #3  
Old 06-05-2007, 01:08 PM
peter
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Default Strong contamination with ethidium bromide

On Jun 4, 6:05 pm, Undergrad <[Only registered users see links. ]> wrote:

Don't worry you will be fine...EtBr has been investigated as a drug
against cancer in the past, not very effective ....
Peter

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  #4  
Old 06-05-2007, 06:40 PM
Jose de las Heras
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Default Strong contamination with ethidium bromide


"Undergrad" <[Only registered users see links. ]> wrote in message
news:[Only registered users see links. ]. net...

what to do?

be a little more careful, and understand what you're handling BEFORE you're
handling it, so that you know what to be careful about, and what to do if
there's an accident.

Other than that, don't worry about your "strong contamination" with EtBr.

Oh, and gloves are useful too ;-)

Jose


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  #5  
Old 06-05-2007, 09:26 PM
Kyle Legate
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Default Strong contamination with ethidium bromide


I'd reply but my fingers are covered with acrylamide dust at the moment.
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  #6  
Old 06-06-2007, 07:50 AM
DK
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Default Strong contamination with ethidium bromide

In article <[Only registered users see links. ]>, Kyle Legate <[Only registered users see links. ]> wrote:

[I realize it's a joke but anyway]

EthBr is clearly overrated but AA's reputation is well-deserved.
Chronic consumption of AA (apparently, above some threshold)
can really can screw you up. In some cases, paralysis occurs and
it is irreversible.

That said, I never wear gloves while pouring gels. Gloves are
abused in today's labs to the point of them being bigger problem
than the problems they are supposed to protect from.

DK
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  #7  
Old 06-14-2007, 03:12 PM
Dr Engelbert Buxbaum
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Default Strong contamination with ethidium bromide



At least one should think about were to use them. Diaminobenzidine for
example is a very fine powder, if you handle an open bottle with gloves
the static electricity will blow it right into your face. But your hands,
of course, are protected.

Removing Eppendorf vials with radioactive samples from a waterbath is
another example. The things are so slippery that you easily splash the
sample across the entire room. I do use gloves when aliquoting radioactive
stocks, but for the few nCi in a sample gloves are indeed more of a
problem than a solution.

To the OP: Before you start working with a substance you should read up on
its properties. Bottle labels and catalogues contain the most important
info like R/S statements. In addition, there are material safety data
sheets (MSDS) available via suppliers web sites. Your lab may also have
printed copies for the chemicals they use (required in some
jurisdictions). There should also be reasonable supervision and training
of new staff members, letting them handle dangerous chemicals without
training is simply criminal.
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