can anyone suggest what "in-frame cloning" means giving me a simple example.
I want to subclone an inser in pCDNA3 to RCAS (A) by adding ClaI sites to
either end. Do I have to worry about keeping the insert "in-frame" in the
RCAS (a viral) vector?
In article <[Only registered users see links. ].net> , "Pragnya Das" <[Only registered users see links. ]> wrote:
"To clone in frame" means to clone in a way that puts your insert
into the same translational frame as the start of the ORF controlled
by promoter under which control you put the expression of your