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| Hi there, I am planning to make a BAC library from a plant species starting with sheared genomic DNA or partially restricted genomic DNA. There is also a company (Epicentre) which provide a kit (pWEB cosmid cloning kit) to make libraries of large genomic inserts (~40 kb) starting from sheared DNA , but it does not seem to be very popular. I have no idea how difficult it is to make such libraries and what kind of problem can hinder the success in making this kind of libraries. Any suggestions/comments/tips? many thanks roberta |
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