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BAC libraries

BAC libraries - Protocols and Methods Forum

BAC libraries - Post Any Protocol, Method, Technique, Procedure or Tips / Troubleshooting for any Molecular Biology Technique.


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Old 05-18-2007, 02:47 PM
Roberta Bergero
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Default BAC libraries



Hi there, I am planning to make a BAC library from a plant species
starting with sheared genomic DNA or partially restricted genomic DNA.
There is also a company (Epicentre) which provide a kit (pWEB cosmid
cloning kit) to make libraries of large genomic inserts (~40 kb)
starting from sheared DNA , but it does not seem to be very popular.
I have no idea how difficult it is to make such libraries and what kind
of problem can hinder the success in making this kind of libraries.
Any suggestions/comments/tips?
many thanks
roberta

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