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immunoprecipitation of protein-RNA complexes

immunoprecipitation of protein-RNA complexes - Protocols and Methods Forum

immunoprecipitation of protein-RNA complexes - Post Any Protocol, Method, Technique, Procedure or Tips / Troubleshooting for any Molecular Biology Technique.


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Old 04-27-2007, 09:27 AM
Lechu
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Default immunoprecipitation of protein-RNA complexes



Hi all,

I look for a protocol for co-immunoprecipitation of protein-RNA
complexes. The target (prey) is RNA which will be detected with RT-
PCR. The RNA-binding protein is fused to GFP and GFP-targeted
antibodies are to be used to precipitate it. I thought of using resin
with covalently coupled antibodies (Pierce Amino-link Gel) but using
protein A/G remains an alternative (a bit less likely to be
successful, but maybe I am wrong). Anyhow, if you know any protocols
or papers that may be relevant I will be grateful for the info, links
and any feedback. Any help is welcome.

Best, Lech

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