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| Hi all, I look for a protocol for co-immunoprecipitation of protein-RNA complexes. The target (prey) is RNA which will be detected with RT- PCR. The RNA-binding protein is fused to GFP and GFP-targeted antibodies are to be used to precipitate it. I thought of using resin with covalently coupled antibodies (Pierce Amino-link Gel) but using protein A/G remains an alternative (a bit less likely to be successful, but maybe I am wrong). Anyhow, if you know any protocols or papers that may be relevant I will be grateful for the info, links and any feedback. Any help is welcome. Best, Lech |
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| complexes , immunoprecipitation , proteinrna |
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