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How important is -80C?

How important is -80C? - Protocols and Methods Forum

How important is -80C? - Post Any Protocol, Method, Technique, Procedure or Tips / Troubleshooting for any Molecular Biology Technique.


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  #1  
Old 04-26-2007, 01:37 PM
Chris Jones
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Default How important is -80C?



We're looking into replacing one of two -80 degree freezers, and the
question came up as to whether we should bother. Currently most of the
contents are bacterial and plasmid stocks as well as some isotopes. It
seems to me that these would fare no worse in a -20 degree box, but I
wondered what experience others have had. What samples "must" be stored
at -80C?

Thanks,

Chris Jones
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Old 04-27-2007, 05:40 PM
Tom Knight
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Default How important is -80C?

Chris Jones <[Only registered users see links. ]> writes:


Bacterial stocks are not stable at -20. Competent cells, in
particular, lose competence quickly at -20, but even glycerol stocks
will suffer over year long periods at -20. Some restriction enzymes
are not stable at -20 and need to be stored at -80. I have no idea
why you are storing isotopes at -80, unless they are sensitive
chemicals. The atoms certainly don't decay any differently depending
on the temperature. It might be possible that radiation damage of the
chemicals is affected.

You want to be sure that your -20 freezers are not frost-free
freezers, which will subject samples to heat/thaw cycles and destroy
living samples very quickly.


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