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make primer solution?

make primer solution? - Protocols and Methods Forum

make primer solution? - Post Any Protocol, Method, Technique, Procedure or Tips / Troubleshooting for any Molecular Biology Technique.


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  #1  
Old 04-25-2007, 02:14 AM
TRAN HO QUANG
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Default make primer solution?



Can netters tell me how to make primer solution from lyophilized (from supplier company) to 100 uM (stock solution). Let say primer has 150 nmoles as recorded from company.

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  #2  
Old 04-25-2007, 07:31 AM
Tim
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Default make primer solution?

Let's see... What are the equations in every textbook?

concentration = moles / liter

In your case:
concentration (you want) = 100 umol / liter
moles (you have) = 150 nmol

turn the equation around to say

liter = moles / concentration

Now get out your calculator and divide

150 nmol / (100 umol / liter)

Make sure you don't drop the unit prefixes nano and micro (n and u).

TRAN HO QUANG wrote:
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  #3  
Old 04-25-2007, 08:30 AM
Duncan Clark
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Default make primer solution?

Historians believe that in newspost
<[Only registered users see links. ].net > on Tue, 24 Apr 2007,
TRAN HO QUANG <[Only registered users see links. ]> penned the following literary
masterpiece:

Strangely I find this simple calculation confuses an awful lot of
people.

I always think like the following:

150nMoles is 150,000pMoles

100uM stock is 100pM per ul

Now use calculator.

Duncan
--
I love deadlines. I especially like the whooshing noise they make as
they go flying by.

Duncan Clark
GeneSys Ltd.
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