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Western blotting problem

Western blotting problem - Protocols and Methods Forum

Western blotting problem - Post Any Protocol, Method, Technique, Procedure or Tips / Troubleshooting for any Molecular Biology Technique.


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Old 04-20-2007, 09:43 PM
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Default Western blotting problem



Hi,



I have some problems on running the SDS PAGE. I found that the western
blot shows higher level of detection on one side and moving gradually
lower to another side. I use the Biorad wet transfer unit at the same
loading of the total proteins.



Thanks a lot,

Sally







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