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PEG Absorbance

PEG Absorbance - Protocols and Methods Forum

PEG Absorbance - Post Any Protocol, Method, Technique, Procedure or Tips / Troubleshooting for any Molecular Biology Technique.


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  #1  
Old 04-19-2007, 06:23 PM
Felipe Leprevost
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Default PEG Absorbance



*Hi all,
*
*Does anyone knows what is the PEG (Polyethilene glycol) absorbance ratios?
I recently used PEG to make a PCR purification and the
spectrophotometry tells me that it** was ok (120 ng/ul . Good 260 measure
and low 280,230 and 320) but **when I loaded the PCR purification into a 1%
Agarose gel it shows me nothing.
I suspect PEG contamination into my sample.

Thanks for any help*
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  #2  
Old 04-20-2007, 03:11 PM
DK
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Default PEG Absorbance

In article <[Only registered users see links. ].net >, "Felipe Leprevost" <[Only registered users see links. ]> wrote:

PEG itself does not absorb ar 260-280. Any absorbance would have
to come from impurities, which are probably variable. What did you
use PEG for? What's 260/280 ratio?

DK


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  #3  
Old 04-20-2007, 04:42 PM
Duncan Clark
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Default PEG Absorbance

Historians believe that in newspost <IK4Wh.853$[Only registered users see links. ]> on
Fri, 20 Apr 2007, DK <[Only registered users see links. ]> penned the following
literary masterpiece:

PEG + NaCl is quite a common method for inexpensively cleaning up a PCR
reaction. It will ppt. the amplified DNA whilst leaving behind primers,
dNTPs and DNA polymerase.

Possibly published by Wayne Barnes in his Klentaq fidelity paper in I
think Gene around 1991-1993. Makes me feel old writing that as I
remember reading it then.

Duncan
--
I love deadlines. I especially like the whooshing noise they make as
they go flying by.

Duncan Clark
GeneSys Ltd.
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  #4  
Old 04-21-2007, 03:23 AM
DK
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Default PEG Absorbance

In article <[Only registered users see links. ].com>, Duncan Clark <[Only registered users see links. ].uk> wrote:

Hey, I used to clean up large alkaline lysis plasmid preps with
PEG for transfections in 1987............... Ouch.

DK


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