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| Dear Aawara, Thanks for your reply! Appreciate it lots Before I ask for anything else, I would like to know if your plasmid (the one with a pSC101 origin) is a promoterless reporter vector? If yes, may I know which reporter gene does it carry? And I've also checked out the pACYC177 and pACYC184 plasmids from NEB. Since they are general cloning vectors (not expression vectors) I think they are not really suitable for my research? (I'm looking into expressing my regulatory protein in higher amounts in hope of them binding to the cloned promoter, so I would basically like an expression vector which carries the T7 promoter.) Cheers, Wai |
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| cells , coexistence , expression , promoterless , reportervector , vector |
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