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Sonication of tributyrin with media chang color to milky

Sonication of tributyrin with media chang color to milky - Protocols and Methods Forum

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Old 04-06-2007, 01:10 PM
muhammad yasir
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Default Sonication of tributyrin with media chang color to milky



I want to check the lipolytic activity of the strain. For this purpose i add tributyrin in media but after sonication the color change to milky. Any one have experince like this and this media will work or not. i shall be very glad to recive ur suggestion
Yasir


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Old 04-07-2007, 02:08 PM
WS
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Default Sonication of tributyrin with media chang color to milky

Dear Muhammad,

that's what you'll get when you sonicate aqueous liquid with oil. You
might be able to break the emulsion by freezing, centrifugation or
adding low viscosity silicon oil or an organic solbent like petrol
ether if your assay permits it.

Wo

On 6 Apr., 15:10, muhammad yasir <[Only registered users see links. ]> wrote:


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