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Gas permeability of silicone oil?

Gas permeability of silicone oil? - Protocols and Methods Forum

Gas permeability of silicone oil? - Post Any Protocol, Method, Technique, Procedure or Tips / Troubleshooting for any Molecular Biology Technique.


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  #1  
Old 03-30-2007, 02:03 PM
Tom Anderson
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Default Gas permeability of silicone oil?



Hi,

Does anyone have any idea how permeable silicone oils are to gases? Oxygen
and carbon dioxide are the ones i'm interested in, really; water, too.

I use a silicone oil to reduce evaporation from dishes i'm looking at on
our inverted scope; my experiments are generally less than an hour long,
so gas exchange isn't a huge deal, but a colleague wants to do some much
longer experiments, and we're not sure if the oil i use is suitable.

My particular oil is a poly(dimethylsiloxane); it's the Dow Corning '200'
type, via Sigma, of 10 centistokes viscosity. I have absolutely no idea
what the degree of the polymer is.

Thanks,
tom

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  #2  
Old 03-30-2007, 04:01 PM
DK
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Default Gas permeability of silicone oil?

In article <[Only registered users see links. ].net >, Tom Anderson <[Only registered users see links. ].uk> wrote:

Evaporation of water through mineral oil (paraffin oil) is very, very slow,
definitely negligible on a timescale of few days even with microliter
drops. Silicone oil MUCH more permeable. In crystallography, silicone
oil is sometimes used to induce very slow drying and mixtures
of various ratios of mineral and silicone are used to finely control
rates of evaporation.

DK

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  #3  
Old 03-30-2007, 04:09 PM
peter
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Default Gas permeability of silicone oil?

On Mar 30, 10:03 am, Tom Anderson <[Only registered users see links. ].uk> wrote:

Hi Tom,
I don't know the answer of the question you asked, but if the
evaporation is an issue, why don't you use humid chamber for long
experiments? I am sure that there are microscopes equipped with small
boxes that keep CO2 and humidity controlled.
my2c, Peter

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  #4  
Old 04-01-2007, 03:39 PM
Tom Anderson
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Default Gas permeability of silicone oil?

On Fri, 30 Mar 2007, peter wrote:


True. The microscope my colleague wants to use has a heated, CO2-gassed
chamber, but it's not humidified. He's going to put a beaker or dish of
water in there, but is still worried about evaporation. We could get a
properly humidifed chamber, but this would involve persuading our
microscope technician, ordering it, fitting it, etc, whereas a suitable
oil is an order to Sigma away!

tom

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Tom Anderson, MRC Laboratory for Molecular Cell Biology, UCL, London WC1E 6BT
(t) +44 (20) 76797264 (f) +44 (20) 76797805 (e) [Only registered users see links. ]
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  #5  
Old 04-01-2007, 04:29 PM
peter
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Default Gas permeability of silicone oil?

On Apr 1, 11:39 am, Tom Anderson <[Only registered users see links. ].uk> wrote:

Hi Tom,
If you already have the heated CO2 chamber on your microscope you can
easily put couple of wet napkins and you will have a nice humidity in
it.
my2c
Peter

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  #6  
Old 04-06-2007, 03:17 PM
Dr Engelbert Buxbaum
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Default Gas permeability of silicone oil?

Tom Anderson wrote:


Thin silicon tubing is used in bioreactors as air/liquid interface to
ensure oxygen supply to the cells.
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  #7  
Old 07-08-2013, 07:19 AM
Pipette Filler
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Default Re: Gas permeability of silicone oil?

Yeah this is good information i like this actually i was in searching for this information and thinking to make a thread for this but i have no need to make any thread after read out this information anyways thanks for this and have a nice day
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