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Safe storage of lipid-membranes for protein lipid overlay assay

Safe storage of lipid-membranes for protein lipid overlay assay - Protocols and Methods Forum

Safe storage of lipid-membranes for protein lipid overlay assay - Post Any Protocol, Method, Technique, Procedure or Tips / Troubleshooting for any Molecular Biology Technique.


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Old 03-27-2007, 08:51 PM
Kyle Legate
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Default Safe storage of lipid-membranes for protein lipid overlay assay



Good day!

I am preparing to perform a protein lipid overlay assay as described by
Dario Alessi et al in the following article:

[Only registered users see links. ]

[Only registered users see links. ]

I would like to prepare several membranes at one time to avoid the
tedium of making serial dilutions of lipid whenever I want to do the
experiment, and I would like opinions on the best way to store them to
keep them fresh? Individually packaged under argon? Floating in blocking
buffer+azide? Stacked in between sheets of blotting paper? Another
clever solution?

Thanks in advance for your ideas.

Kyle
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Old 03-28-2007, 09:26 AM
Aawara Chowdhury
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Default Safe storage of lipid-membranes for protein lipid overlay assay

In <[Only registered users see links. ]>,
Kyle Legate <[Only registered users see links. ]> wrote:


Not exactly the same thing, but one of my students stored DRMs ("lipid
rafts") from virus-infected cells under nitrogen (in whatever
concentration of TX-100 the DRM extraction was done in.

AC
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assay , lipid , lipidmembranes , overlay , protein , safe , storage


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