In <email@example.com. com>,
peter <[Only registered users see links. ]> wrote:
Oligonucleotide purity is typically assessed by MALDI-TOF mass
spec, or electrospray ionization mass spec (ESI). There is a
relatively recent paper, titled "High-throughput analysis of
oligonucleotides using automated electrospray ionization mass
spectrometry", by M.E. Hail et al. that compares the two
techniques (Am. Biotech. Lab 22: 12-24).
ESI was reliable for oligonucleotides upto 120 nt in length.
MALDI-TOF was reliable up to about 50 nt.
Email: echo 36434455860060025978157675027927670979097959886449 930P | dc
On Mar 22, 8:01 am, Aawara Chowdhury <[Only registered users see links. ]> wrote:
ESI and MALDI is usually provided by the supplier. I want to know
minor (less than 1%) fraction that might carry mutations. I was
thinking about sequencing, but that is quite brutal force and won't
give me results easily. Maybe I would make a construct with out of
frame AbtR and then I will clone my oligo (keeping AbtR out of frame)
and plate on antibiotic to see how many clones are resistant (e.g.
having oligoes that have 1bp deletions and repair the frame). Any