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| Dear all, I have been trying to use cycloheximide to inhibit protein synthesis in stably transfected Hek293 cells. I tried 0.2-20ug/ml and my cells died after 8 hrs incubation in 1-20ug/ml. At lower conc, there is no inhibition. Does anyone know why and if I can try other inhibitors? Thanks. __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around [Only registered users see links. ] |
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