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cycloheximide conc

cycloheximide conc - Protocols and Methods Forum

cycloheximide conc - Post Any Protocol, Method, Technique, Procedure or Tips / Troubleshooting for any Molecular Biology Technique.


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Old 03-05-2007, 01:41 AM
lam
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Default cycloheximide conc



Dear all,
I have been trying to use cycloheximide to inhibit
protein synthesis in stably transfected Hek293 cells.
I tried 0.2-20ug/ml and my cells died after 8 hrs
incubation in 1-20ug/ml. At lower conc, there is no
inhibition.
Does anyone know why and if I can try other
inhibitors?

Thanks.

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