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Inert Dye for protein solution

Inert Dye for protein solution - Protocols and Methods Forum

Inert Dye for protein solution - Post Any Protocol, Method, Technique, Procedure or Tips / Troubleshooting for any Molecular Biology Technique.


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Old 03-01-2007, 03:52 PM
Tim Fitzwater
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Default Inert Dye for protein solution




There are published reports of two different red dyes used for mixing with Taq DNA polymerase, and commercial versions are available.

1) 0.01% Phenol red (R. J. Klebe, S. A. Rodriguez, M. L. VerBeek and T. A. Giambernardi, 1999. Simple method for "hot-start" RT-PCR. BioTechn. 27(6):1108-1110.)

2) Carl Wittwer's laboratory uses 0.1 mM Cresol Red for the same purpose (The Rapid Cyclist, Spring, 1994, page 9).

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