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#1
03-01-2007, 02:52 PM
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 Inert Dye for protein solution There are published reports of two different red dyes used for mixing with Taq DNA polymerase, and commercial versions are available. 1) 0.01% Phenol red (R. J. Klebe, S. A. Rodriguez, M. L. VerBeek and T. A. Giambernardi, 1999. Simple method for "hot-start" RT-PCR. BioTechn. 27(6):1108-1110.) 2) Carl Wittwer's laboratory uses 0.1 mM Cresol Red for the same purpose (The Rapid Cyclist, Spring, 1994, page 9).  |
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