Go Back   Science Forums Biology Forum Molecular Biology Forum Physics Chemistry Forum > Molecular Research Topics Forum > Protocols and Methods Forum
Register Search Today's Posts Mark Forums Read

Protocols and Methods Forum Post Any Protocol, Method, Technique, Procedure or Tips / Troubleshooting for any Molecular Biology Technique.


Purifying PCR product from 2% agarose gel

Purifying PCR product from 2% agarose gel - Protocols and Methods Forum

Purifying PCR product from 2% agarose gel - Post Any Protocol, Method, Technique, Procedure or Tips / Troubleshooting for any Molecular Biology Technique.


Reply
 
LinkBack Thread Tools Display Modes
  #1  
Old 02-20-2007, 06:04 PM
Yoram Gerchman
Guest
 
Posts: n/a
Default Purifying PCR product from 2% agarose gel




I have noticed Qiagene kits (as well as others) behave badly when the wash
buffer lose its EtOH (like by evaporation. Its enough to have someone leave the
cap not tight to have this happen. Try to add some 100% EtOH to the wash buffer.
Yoram


------------------------------------------------------------------------
This message was sent using IMP, the Webmail Program of Haifa University

Reply With Quote
  #2  
Old 02-21-2007, 11:47 PM
Jose de las Heras
Guest
 
Posts: n/a
Default Purifying PCR product from 2% agarose gel


"Yoram Gerchman" <[Only registered users see links. ].il> wrote in message
news:[Only registered users see links. ].ne t...

?

The wash buffer is 80% EtOH... I would imagine that an awful lot of
evaporation will have to occur before it becomes a problem.

Jose


Reply With Quote
Reply

Tags
agarose , gel , pcr , product , purifying


Thread Tools
Display Modes

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off
Trackbacks are On
Pingbacks are On
Refbacks are On

Forum Jump

Similar Threads
Thread Thread Starter Forum Replies Last Post
Purifying PCR product from 2% agarose gel hilary cassidy Protocols and Methods Forum 5 02-23-2007 11:08 AM
Purifying PCR product from 2% agarose gel Tom Anderson Protocols and Methods Forum 0 02-19-2007 08:44 PM
Purifying PCR product from 2% agarose gel (by Paco) Francisco Roberto Quiroz Figueroa Protocols and Methods Forum 0 02-18-2007 07:38 PM
Nusieve agarose gel Samantha Protocols and Methods Forum 1 11-05-2004 10:32 AM


All times are GMT. The time now is 12:04 AM.


Powered by vBulletin® Version 3.8.4
Copyright ©2000 - 2014, Jelsoft Enterprises Ltd.
Copyright 2005 - 2012 Molecular Station | All Rights Reserved
Page generated in 0.11528 seconds with 16 queries