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Fixation for PIP2 immunofluorescence

Fixation for PIP2 immunofluorescence - Protocols and Methods Forum

Fixation for PIP2 immunofluorescence - Post Any Protocol, Method, Technique, Procedure or Tips / Troubleshooting for any Molecular Biology Technique.


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Old 01-16-2007, 02:47 PM
Tom Anderson
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Default Fixation for PIP2 immunofluorescence



Hi,

Has anyone done any immunofluorescence against PIP2? If so, how did you
fix the cells?

I have a protocol involving a three-hour formaldehyde/glutaraldehyde
double fixation on ice, but if it was possible to do it at room
temperature or 37 C, at least to begin with, that would be a lot better
for the cells.

Thanks,
tom

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Tom Anderson, MRC Laboratory for Molecular Cell Biology, UCL, London WC1E 6BT
(t) +44 (20) 76797264 (f) +44 (20) 76797805 (e) [Only registered users see links. ]

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