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#1
01-16-2007, 01:47 PM
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 Fixation for PIP2 immunofluorescence Hi, Has anyone done any immunofluorescence against PIP2? If so, how did you fix the cells? I have a protocol involving a three-hour formaldehyde/glutaraldehyde double fixation on ice, but if it was possible to do it at room temperature or 37 C, at least to begin with, that would be a lot better for the cells. Thanks, tom -- Tom Anderson, MRC Laboratory for Molecular Cell Biology, UCL, London WC1E 6BT (t) +44 (20) 76797264 (f) +44 (20) 76797805 (e) [Only registered users see links. ]  |
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| fixation , immunofluorescence , pip2 |
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