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Subject: Blocking biotin labeled blots with BSA?

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Subject: Blocking biotin labeled blots with BSA?

Subject: Blocking biotin labeled blots with BSA? - Protocols and Methods Forum

Subject: Blocking biotin labeled blots with BSA? - Post Any Protocol, Method, Technique, Procedure or Tips / Troubleshooting for any Molecular Biology Technique.

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01-11-2007, 06:34 PM

Steve Crampton

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Subject: Blocking biotin labeled blots with BSA?

Hi Wolfgang,
If you are worried about background and are not sure if there is biotin in
your BSA, Why don't you just take a blank membrane, block it with BSA, and
incubate with the peroxidase to see if you have background or not. You
would not have to waste your samples on a test.
Best regards
Steve Crampton
Molecular Biology & Biochemistry
University of California, Irvine
3113 McGaugh Hall
Irvine, CA 92697-3900
lab (949) 824-8143
fax (949) 824-8551
[Only registered users see links. ]

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Subject: Blocking biotin labeled blots with BSA?

Subject: Blocking biotin labeled blots with BSA? - Protocols and Methods Forum

Subject: Blocking biotin labeled blots with BSA? - Post Any Protocol, Method, Technique, Procedure or Tips / Troubleshooting for any Molecular Biology Technique.