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#1
12-16-2006, 04:54 PM
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 non specific amplification in clone fragment using primer aganist vector Dear Muhammad, Possible reasons: Problem with the insert or problem with the primers. Do you have any unrelated fragment in the same vector where you know that the insert is ok? Did you try a gradient PCR? Do you get the same band when you employ just 1 primer in the rxn? Can you cut out the insert and check its length? Wo muhammad yasir wrote:  |
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| aganist , amplification , clone , fragment , primer , specific , vector |
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