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non specific amplification in clone fragment using primer aganist vector

non specific amplification in clone fragment using primer aganist vector - Protocols and Methods Forum

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Old 12-16-2006, 05:54 PM
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Default non specific amplification in clone fragment using primer aganist vector



Dear Muhammad,

Possible reasons: Problem with the insert or problem with the primers.

Do you have any unrelated fragment in the same vector where you know
that the insert is ok?
Did you try a gradient PCR?
Do you get the same band when you employ just 1 primer in the rxn?
Can you cut out the insert and check its length?

Wo


muhammad yasir wrote:

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aganist , amplification , clone , fragment , primer , specific , vector


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