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question about making sodium ortho-vanadate stock solution

question about making sodium ortho-vanadate stock solution - Protocols and Methods Forum

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  #1  
Old 12-11-2006, 06:42 PM
Z.L. K
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Default question about making sodium ortho-vanadate stock solution



I checked some online recipes about how to make sodium ortho-vanadate
stock solution, some talk about pH-ing the solution first after the
reagents completely dissolve in water, which would then turn to yellow
after pH-ing to 10, then the solution needs to be boiled to get rid of
this color, this process (pH-ing then boiling) need to be repeated a
few cycles until the color is gone after pH to 10. However, some other
recipes do not mention this at all. Since quite a significant amout of
recipes I checked online emphasize this step, and I really don't want
to make a wrong solution for my experiment, I would like to ask if
anybody knows why one needs to (or IF one needs to) pH-ing and boiling
the solution when preparing sodium ortho-vanadate stock? I came across
to a line online saying this would help to activate the chemical
compound, however all the websites I checked, including vendor
websites who sell this compound, do not mention anything regarding
this either. Thanks if you can help me to straight this out!

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  #2  
Old 12-13-2006, 03:11 PM
Dr Engelbert Buxbaum
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Default question about making sodium ortho-vanadate stock solution

Z.L. K wrote:


For which purpose do you need the solution? For phosphate determination
boiling is not required.
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  #3  
Old 09-17-2009, 04:25 PM
Pipette Filler
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Default Re: question about making sodium ortho-vanadate stock solution

This site says that the boiling makes the vanadate a more potent inhibitor of phosphatases
www signaling-gateway org/data/cgi-bin/ProtocolFile.cgi/afcs_PS00000307.pdf?pid=PS00000307
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Old 09-18-2009, 09:33 AM
Pipette Filler
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Default Re: question about making sodium ortho-vanadate stock solution

sodium orthvandate : inhibitor of phosphatases~
I just prepare the solution as:
Dissolve the sodium orthvandate in ddw (100mM or 200mM), adjust pH to 10 using HCl, the solution may turns yellow,boil the solution until it turns to colorless~repeat the cycle for several times until the solution still colorless and pH stablized at 10. you may store the aliquots at -20c.
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Old 10-20-2009, 12:26 PM
Pipette Filler
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Default Re: question about making sodium ortho-vanadate stock solution

Hi,

Do you guys know how to prepare sodium orthovanadate for inhibition of dynein? Is it the same as mentioned above?

Thanks.
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