question about making sodium ortho-vanadate stock solution

I checked some online recipes about how to make sodium ortho-vanadate
stock solution, some talk about pH-ing the solution first after the
reagents completely dissolve in water, which would then turn to yellow
after pH-ing to 10, then the solution needs to be boiled to get rid of
this color, this process (pH-ing then boiling) need to be repeated a
few cycles until the color is gone after pH to 10. However, some other
recipes do not mention this at all. Since quite a significant amout of
recipes I checked online emphasize this step, and I really don't want
to make a wrong solution for my experiment, I would like to ask if
anybody knows why one needs to (or IF one needs to) pH-ing and boiling
the solution when preparing sodium ortho-vanadate stock? I came across
to a line online saying this would help to activate the chemical
compound, however all the websites I checked, including vendor
websites who sell this compound, do not mention anything regarding
this either. Thanks if you can help me to straight this out!

Z.L. K wrote:


For which purpose do you need the solution? For phosphate determination
boiling is not required.

[seemin]

This site says that the boiling makes the vanadate a more potent inhibitor of phosphatases
www signaling-gateway org/data/cgi-bin/ProtocolFile.cgi/afcs_PS00000307.pdf?pid=PS00000307

[Archonster]

sodium orthvandate : inhibitor of phosphatases~
I just prepare the solution as:
Dissolve the sodium orthvandate in ddw (100mM or 200mM), adjust pH to 10 using HCl, the solution may turns yellow,boil the solution until it turns to colorless~repeat the cycle for several times until the solution still colorless and pH stablized at 10. you may store the aliquots at -20c.

[rlong]

Hi,

Do you guys know how to prepare sodium orthovanadate for inhibition of dynein? Is it the same as mentioned above?

Thanks.