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Methods Digest, Vol 19, Issue 7 to Lechu Casein blocking question

Methods Digest, Vol 19, Issue 7 to Lechu Casein blocking question - Protocols and Methods Forum

Methods Digest, Vol 19, Issue 7 to Lechu Casein blocking question - Post Any Protocol, Method, Technique, Procedure or Tips / Troubleshooting for any Molecular Biology Technique.


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Old 12-09-2006, 05:51 AM
Jess
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Default Methods Digest, Vol 19, Issue 7 to Lechu Casein blocking question







Message: 8
Date: 8 Dec 2006 02:48:57 -0800
From: "Lechu"
Subject: [western blotting] Abcam's ab9484 b-actin loading control vs
casein blocking
To: [Only registered users see links. ]
Message-ID: <1165574937.240910.232870@16g2000cwy.googlegroups. com>
Content-Type: text/plain; charset="iso-8859-2"

Hi all,

Abcam strongly reccommend to block ab9484 b-actin loading control
antibody (ab9484) with BSA instead of milk. Does anyone know what milk
component is most likely to cause problems with this ab? I need to
block this antibody with casein, hence my question.

best, Lech

Hi Lech,

Check this paper- looks like they used your Abcam ab and blocked with casein... (Sorry I can't get the whole paper from home). Hope this is useful to you.

Cheers,
Jess

Excerpt:

150 ll of the blocking solution consisting of 1% casein in ... for b-actin (Abcam) as a loading control and for normalization ...





Journal of Neuroscience Research Volume 84, Issue 8 , Pages 1871 - 1878Published Online: 22 Sep 2006




setDOI("ADOI=10.1002/jnr.21074"); setISSN("1097-4547") setISSN("0360-4012") setEarlyView("-----") Research Article
Lack of neprilysin suffices to generate murine amyloid-like deposits in the brain and behavioral deficit in vivo
Rime Madani 1, Raphael Poirier 2, David P. Wolfer 1, Hans Welzl 1, Peter Groscurth 1, Hans-Peter Lipp 1, Bao Lu 3, Mohammed El Mouedden 4, Marc Mercken 4, Roger M. Nitsch 2, M. Hasan Mohajeri 2 *




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