| |||||||
| Register | Search | Today's Posts | Mark Forums Read |
| Protocols and Methods Forum Post Any Protocol, Method, Technique, Procedure or Tips / Troubleshooting for any Molecular Biology Technique. |
 |
| | LinkBack | Thread Tools | Display Modes |
|
#1
12-06-2006, 06:08 PM
| |||
| |||
 I am looking for a protocol to purify DNA drom silver stained PAGEGels Hello, i am doing my own sizestandart so I can make it with the exact band sizes I have and I would like to find a way to purify the DNA I run in a 8% polyacrilamid Gel, colored with silver. The thing is that I am going to use this DNA into a PCR reaction and I think silver will compete with magnesium In the reaction giving me a bad reaction and low DNA. Is there any nice protocol to extract the DNA from the Gel and get rid of the Silver ? or the silver doesn't compete with Mg ? Thanks Cristiano Fontes  |
|
| Tags |
| dna , drom , pagegels , protocol , purify , silver , stained |
| Thread Tools | |
| Display Modes | |
|
|
Similar Threads | ||||
| Thread | Thread Starter | Forum | Replies | Last Post |
| silver stained band at the running front | Monica Fazzini | Protein Forum | 1 | 02-21-2012 05:23 AM |
| Silver stained 2-d gel BLOB!!! | sam_boooko | 2-D Gel Electrophoresis Forum | 5 | 06-02-2011 04:19 PM |
| good morning | Tim Fitzwater | Protocols and Methods Forum | 0 | 04-16-2008 02:21 PM |
| silver stained band at the running front (Monica Fazzini) | Ligong Wang | Protein Forum | 0 | 10-23-2007 06:22 PM |
| Silver stained gels - background problem when using DTT | Amanda | Protocols and Methods Forum | 5 | 01-29-2004 03:31 PM |
Linear Mode
