Go Back   Science Forums Biology Forum Molecular Biology Forum Physics Chemistry Forum > Molecular Research Topics Forum > Protocols and Methods Forum
Register Search Today's Posts Mark Forums Read

Protocols and Methods Forum Post Any Protocol, Method, Technique, Procedure or Tips / Troubleshooting for any Molecular Biology Technique.


I am looking for a protocol to purify DNA drom silver stained PAGEGels

I am looking for a protocol to purify DNA drom silver stained PAGEGels - Protocols and Methods Forum

I am looking for a protocol to purify DNA drom silver stained PAGEGels - Post Any Protocol, Method, Technique, Procedure or Tips / Troubleshooting for any Molecular Biology Technique.


Reply
 
LinkBack Thread Tools Display Modes
  #1  
Old 12-06-2006, 07:08 PM
Cristiano Fontes
Guest
 
Posts: n/a
Default I am looking for a protocol to purify DNA drom silver stained PAGEGels



Hello, i am doing my own sizestandart so I can make it with the exact band
sizes I have and I would like to find a way to purify the DNA I run in a 8%
polyacrilamid Gel, colored with silver. The thing is that I am going to use
this DNA into a PCR reaction and I think silver will compete with magnesium
In the reaction giving me a bad reaction and low DNA.

Is there any nice protocol to extract the DNA from the Gel and get rid of
the Silver ? or the silver doesn't compete with Mg ?

Thanks

Cristiano Fontes
Reply With Quote
Reply

Tags
dna , drom , pagegels , protocol , purify , silver , stained


Thread Tools
Display Modes

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off
Trackbacks are On
Pingbacks are On
Refbacks are On

Forum Jump

Similar Threads
Thread Thread Starter Forum Replies Last Post
silver stained band at the running front Monica Fazzini Protein Forum 1 02-21-2012 06:23 AM
Silver stained 2-d gel BLOB!!! sam_boooko 2-D Gel Electrophoresis Forum 5 06-02-2011 04:19 PM
good morning Tim Fitzwater Protocols and Methods Forum 0 04-16-2008 02:21 PM
silver stained band at the running front (Monica Fazzini) Ligong Wang Protein Forum 0 10-23-2007 06:22 PM
Silver stained gels - background problem when using DTT Amanda Protocols and Methods Forum 5 01-29-2004 04:31 PM


All times are GMT. The time now is 09:49 PM.


Powered by vBulletin® Version 3.8.4
Copyright ©2000 - 2014, Jelsoft Enterprises Ltd.
Copyright 2005 - 2012 Molecular Station | All Rights Reserved
Page generated in 0.10927 seconds with 16 queries