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Qiagen buffer PN

Qiagen buffer PN - Protocols and Methods Forum

Qiagen buffer PN - Post Any Protocol, Method, Technique, Procedure or Tips / Troubleshooting for any Molecular Biology Technique.

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Old 11-19-2006, 10:12 PM
Daniel Ciznadija
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Default Qiagen buffer PN

Dear all,

I'd like to use a microspin column to purify 38-50bp oligonucleotide fromexcess nucleotides and enzyme. The Qiaquik columns from Qiagen seem ideal for this, but a critical buffer is lacking (a buffer designated "PN").This buffer apparently contains sodium perchlorate. I'd like to use this buffer, but would prefer not to have to spend large bundles of money for a bulk solution when this is really a one-pot shot. Does anyone know the recipe for this buffer or of a similar buffer for my purposes?

Thanks in advance for the help,

Daniel Ciznadija

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