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#1
11-05-2006, 04:03 PM
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 VSMCs extraction Dear Dr Richard Hastings, I am a PhD student from Greece trying desperately to extract VSMC from carotid artery.Unfortunately,I do not have an answer to your problem, but I was wondering if you can help me with mine.You seem to have managed to extract the cells from the vessel, while I am trying with no result.I use a protocol based on collagenase digestion of carotids pieces in serum free DMEM for 2 hours and then cultivation in DMEM+FCS 10%.I use gelatin-coated plates and change the medium every 72 hours.What am I doing wrong?II have tried other relevant protocols but it still doesnt work for me.Couldyou please help me by mailing your protocol?If this is not possible any answer would be a great help for me! Thank you in advance. Voula Savvari  |
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| extraction , vsmcs |
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