Go Back   Science Forums Biology Forum Molecular Biology Forum Physics Chemistry Forum > Molecular Research Topics Forum > Protocols and Methods Forum
Register Search Today's Posts Mark Forums Read

Protocols and Methods Forum Post Any Protocol, Method, Technique, Procedure or Tips / Troubleshooting for any Molecular Biology Technique.

VSMCs extraction

VSMCs extraction - Protocols and Methods Forum

VSMCs extraction - Post Any Protocol, Method, Technique, Procedure or Tips / Troubleshooting for any Molecular Biology Technique.

LinkBack Thread Tools Display Modes
Old 11-05-2006, 05:03 PM
Voula Savvari
Posts: n/a
Default VSMCs extraction

Dear Dr Richard Hastings,
I am a PhD student from Greece trying desperately to extract VSMC from
carotid artery.Unfortunately,I do not have an answer to your problem, but I
was wondering if you can help me with mine.You seem to have managed to
extract the cells from the vessel, while I am trying with no result.I use a
protocol based on collagenase digestion of carotids pieces in serum free
DMEM for 2 hours and then cultivation in DMEM+FCS 10%.I use gelatin-coated
plates and change the medium every 72 hours.What am I doing wrong?II
have tried other relevant protocols but it still doesnt work for
me.Couldyou please help me by mailing your protocol?If this is not
possible any
answer would be a great help for me!

Thank you in advance.

Voula Savvari
Reply With Quote

extraction , vsmcs

Thread Tools
Display Modes

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off
Trackbacks are On
Pingbacks are On
Refbacks are On

Forum Jump

Similar Threads
Thread Thread Starter Forum Replies Last Post
Problem met in carotenoid extraction angel00123 Protocols and Methods Forum 2 05-20-2010 04:46 AM
DNA EXTRACTION Dunowska, Magda Protocols and Methods Forum 0 05-21-2008 12:07 AM
Plant Protein Extraction the Hard Way danfive Protein Science 0 04-24-2008 08:53 PM
Protein extraction and RNA extraction Sami Zebrafish Forum 0 03-10-2006 03:33 PM
DNA extraction from bacteria on activated carbon Chen Xiaoge Microbiology Forum 0 08-07-2003 01:32 AM

All times are GMT. The time now is 08:10 PM.

Powered by vBulletin® Version 3.8.4
Copyright ©2000 - 2015, Jelsoft Enterprises Ltd.
Copyright 2005 - 2012 Molecular Station | All Rights Reserved
Page generated in 0.11029 seconds with 16 queries