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#1
11-01-2006, 11:49 AM
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 Beta-galactosidase detection Hi, I'm having difficulties with my immunohistochemistry. I want to show Beta-galactosidase on cryo-slides using the Promega Anti-Beta-gal antibody. It does not work, the antibody does detect beta-galactosidase. But when using it on cryo-tissue (10 micron) I don't get a clear signal and a lot of background. I used thicker slides (100 micron) and even then the signal is low. Can anybody help me with this problem? Thanks Geert van Tetering -- Geert van Tetering PhD student Molecular Research Lab Dept. of Pathology UMCU The Netherlands  |
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| betagalactosidase , detection |
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