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Beta-galactosidase detection

Beta-galactosidase detection - Protocols and Methods Forum

Beta-galactosidase detection - Post Any Protocol, Method, Technique, Procedure or Tips / Troubleshooting for any Molecular Biology Technique.


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Old 11-01-2006, 12:49 PM
Tetering, G.J. van
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Default Beta-galactosidase detection



Hi,

I'm having difficulties with my immunohistochemistry. I want to show
Beta-galactosidase on cryo-slides using the Promega Anti-Beta-gal
antibody. It does not work, the antibody does detect beta-galactosidase.
But when using it on cryo-tissue (10 micron) I don't get a clear signal
and a lot of background. I used thicker slides (100 micron) and even
then the signal is low. Can anybody help me with this problem?

Thanks
Geert van Tetering

--

Geert van Tetering
PhD student
Molecular Research Lab
Dept. of Pathology UMCU
The Netherlands

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