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What does the color of my protein solution mean?

What does the color of my protein solution mean? - Protocols and Methods Forum

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  #1  
Old 10-27-2006, 03:46 AM
Yang
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Default What does the color of my protein solution mean?



Hi,guys! I need your help!
I recently purified a protein. It is in yellow color.
What does the color mean?
Thank you in advance!

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  #2  
Old 10-27-2006, 02:30 PM
Domo
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Default What does the color of my protein solution mean?

got HNO3?
some acid? - neutralise using NaOH

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  #3  
Old 10-27-2006, 03:40 PM
Nick Theodorakis
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Default What does the color of my protein solution mean?


Yang wrote:

If it's pure it might mean it has a prosthetic group, such as a flavin
or a rhodopsin.

Did you do a spectrum?

Nick

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  #4  
Old 10-29-2006, 02:20 PM
Yang
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Default What does the color of my protein solution mean?

Thanks a lot !
I deal with a normal protein without what you mentioned before.
I am really puzzuled.





Nick Theodorakis wrote:

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  #5  
Old 10-30-2006, 06:18 AM
Oliver Berkowitz
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Default What does the color of my protein solution mean?

Hi,

maybe a cofactor like pyridoxal phosphate. Had a yellow protein with that as
well...

Cheers
Oliver

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  #6  
Old 10-30-2006, 04:02 PM
Pow Joshi
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Default What does the color of my protein solution mean?

On 10/27/06, Domo <rimask@yahoo.com> wrote:

hi,

was wondering what your starting material was...... long time ago we
had a blue protein, and it did'nt mean a thing...atleast not to me )

best
pow

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  #7  
Old 10-30-2006, 04:06 PM
Pow Joshi
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Default What does the color of my protein solution mean?

On 10/30/06, Pow Joshi <[Only registered users see links. ]> wrote:

....adding to my previous comment, if it's quite concentrated a
fraction (it will be frothing a little as well), it might apprear as a
pale yellow fluid ...(eg: albumin ) ..... it's just an observation
.....



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  #8  
Old 10-30-2006, 06:24 PM
Carlo Zambonelli
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Default What does the color of my protein solution mean?

A "blue" protein most likely contains a metal cofactor (i.e. iron)
coordinated to Tyr and possibly Asp or Glu and Cys.
Carlo

On 10/30/06, Pow Joshi <[Only registered users see links. ]> wrote:
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  #9  
Old 10-30-2006, 07:15 PM
Pow Joshi
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Default What does the color of my protein solution mean?

On 10/30/06, Carlo Zambonelli <[Only registered users see links. ]> wrote:
sure, and that's what we fancied .... however, wonder what kind of
blue protein exists in the mammalian serum....

thanks
pow

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  #10  
Old 10-30-2006, 07:58 PM
Carlo Zambonelli
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Default What does the color of my protein solution mean?

Google quickly answers your question with at least one example:
ceruloplasmin (color comes from Cu binding). I would bet more proteins
(involved in Fe transport?) are out there.

On 10/30/06, Pow Joshi <[Only registered users see links. ]> wrote:
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