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low molarity borate and acetate agarose gels

low molarity borate and acetate agarose gels - Protocols and Methods Forum

low molarity borate and acetate agarose gels - Post Any Protocol, Method, Technique, Procedure or Tips / Troubleshooting for any Molecular Biology Technique.


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Old 10-23-2006, 08:41 PM
Pow Joshi
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Default low molarity borate and acetate agarose gels



Hi,

frankly, I had run, regularly, some TBE gels (tris borate EDTA) and
the protocol is available in the Molecular cloning book, especially
the old editions by Tom Maniatis.....
You could use 0.5X instead of 1x buffer; alternatively, you could use
low voltages.
I used to find the resolutions comparable .... although it's easy to
run TAE gels if you wish to isolate/ purify your DNA.

hope this helps

pow
On 10/19/06, Ho-Leung Ng <[Only registered users see links. ]> wrote:

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