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Concentrating adenovirus by ultracentrifugation

Concentrating adenovirus by ultracentrifugation - Protocols and Methods Forum

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Old 10-19-2006, 02:23 AM
Zhonglin Chai
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Default Concentrating adenovirus by ultracentrifugation



Normally we purify (and concentrate, of course) adenovirus by double CsCl banding.

Zhonglin Chai


-----Original Message-----
From: [Only registered users see links. ]
[mailto:[Only registered users see links. ].indiana.edu]On Behalf Of Tom Anderson
Sent: Thursday, 19 October 2006 8:57 AM
To: Ye Olde Methods and Reagents List
Subject: Concentrating adenovirus by ultracentrifugation


Evening all,

Has anyone here concentrated adenoviruses, or any other kind of virus or
similar, by ultracentrifugation? If so, what product did you use, and in
particular, what pore size of membrane?

I'm doing this reasonably successfully using 100 kDa MWCO Amicon Ultras;
however, i get a lot of junk in my retentate, which i assume is serum
proteins, so i'd like to move to a larger pore size, to let that through.
However, i have no idea what molecular mass-based pore sizes mean in
nanometre terms; the various manufacturers' websites are not a lot of use
(notable exception: Pall [1]).

Thanks,
tom

[1] [Only registered users see links. ]

--
Tom Anderson, MRC Laboratory for Molecular Cell Biology, UCL, London WC1E 6BT
(t) +44 (20) 76797264 (f) +44 (20) 76797805 (e) [Only registered users see links. ]

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