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Ammonium bicarbonate in IE chromatography

Ammonium bicarbonate in IE chromatography - Protocols and Methods Forum

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  #1  
Old 08-31-2006, 10:32 AM
pawel.stocki@ncl.ac.uk
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Default Ammonium bicarbonate in IE chromatography



Hi,
Does anyone have some experience in using ammonium bicarbonate buffer
for gradient elution from Q-Sepharose or other IE? I would like to use
it instead of NaCl gradient. The sample will be subjected for further
MS analysis that is why I would like to use volatile salt. I'll
appreciate any comments.

Thanks,
Pawel

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Old 09-01-2006, 02:35 AM
DK
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Default Ammonium bicarbonate in IE chromatography

In article <1157020336.038624.230310@h48g2000cwc.googlegroups .com>, [Only registered users see links. ] wrote:

Should be no problem at all. It's a commonly used volatile salt
and it is quite gentle to proteins. Bicarbonate is slighly more
active chloride so be prepared to elution at slightly lower salt.

DK




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