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protein sticking to membranes

protein sticking to membranes - Protocols and Methods Forum

protein sticking to membranes - Post Any Protocol, Method, Technique, Procedure or Tips / Troubleshooting for any Molecular Biology Technique.


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Old 08-30-2006, 03:37 PM
Maryam
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Default protein sticking to membranes



In our lab we pretreat the centricon with 1% Human serum albumin overnight before adding our protein and we get good recovery.

Ho-Leung Ng <[Only registered users see links. ]> wrote: Hello,

I am working with a protein that sticks to membranes used in dialysis
and concentrators. I've tried both PES and cellulose membranes. This
makes buffer exchange and concentrating a huge pain. The protein is
well behaved in solution, and the stickiness is not due to
aggregation. I can recover some of my protein by washing the membrane
afterward with buffer, but the yield is not great and inconsistent.
Are there other membranes worth trying? Does anyone else have
experience with sticky proteins?


Thank you,
ho

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