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is it possible to stain a dried polyacrylamide gel?

is it possible to stain a dried polyacrylamide gel? - Protocols and Methods Forum

is it possible to stain a dried polyacrylamide gel? - Post Any Protocol, Method, Technique, Procedure or Tips / Troubleshooting for any Molecular Biology Technique.


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  #1  
Old 08-28-2006, 01:03 AM
jeremyjz@gmail.com
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Default is it possible to stain a dried polyacrylamide gel?



Hi,

I have a 15% SDS-polyacrylamide gel that has been dried using a gel
dryer (2hr at 80C) some days ago. At the time I did not stain the gel
before drying as it was intended for autoradiography. However, now I
would like to stain the gel so that I would know for sure whether the
radiolabeled band on the film co-migrates with my protein of interest
that can be easily detected by coomassie blue. Does anyone know how to
stain a dried gel or has anyone done it? The dried gel is currently on
a filter paper (which was used during drying). Any helpful comments
will be appreciated.

Thanks very much.

Jeremy

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  #2  
Old 08-28-2006, 02:39 AM
DK
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Default is it possible to stain a dried polyacrylamide gel?

In article <1156727021.083026.315290@m73g2000cwd.googlegroups .com>, [Only registered users see links. ] wrote:

Not sure about filter paper... Other than that, gels dried between
cellophane sheets can be simply rehydrated in water, stained with
whatever reagent (I tried silver after drying post-Coomassie) and
even used (with lowered sensitivity) in Westerns.

DK



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  #3  
Old 08-28-2006, 09:09 PM
Pow Joshi
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Default is it possible to stain a dried polyacrylamide gel?

Does anyone know how to
stain a dried gel or has anyone done it? The dried gel is currently on
a filter paper (which was used during drying). Any helpful comments
will be appreciated.

if you place the gel with the filter paper in water or fixative, and
then gently remove the filter paper, when the gel rehydrates and
separates, it should work ...... the gel is rather vulnerable to
beakage when it is not fully rehydrated ...that's teh onbly care you
need to take.

pow

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  #4  
Old 08-29-2006, 08:45 AM
Dr Engelbert Buxbaum
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Default is it possible to stain a dried polyacrylamide gel?

DK wrote:



I agree, drying between cellophane has considerable advantages over the
filter paper method. But rehydration is possible also for gels dried on
filter paper. In both cases it helps to add 5-10% glycerol to the water.
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