I have a 15% SDS-polyacrylamide gel that has been dried using a gel
dryer (2hr at 80C) some days ago. At the time I did not stain the gel
before drying as it was intended for autoradiography. However, now I
would like to stain the gel so that I would know for sure whether the
radiolabeled band on the film co-migrates with my protein of interest
that can be easily detected by coomassie blue. Does anyone know how to
stain a dried gel or has anyone done it? The dried gel is currently on
a filter paper (which was used during drying). Any helpful comments
will be appreciated.
Thanks very much.