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thiazole orange AND ssDNA

thiazole orange AND ssDNA - Protocols and Methods Forum

thiazole orange AND ssDNA - Post Any Protocol, Method, Technique, Procedure or Tips / Troubleshooting for any Molecular Biology Technique.


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  #1  
Old 07-26-2006, 12:23 AM
Ted
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Default thiazole orange AND ssDNA




Hi, all!
I have a question about DNA dyeing. Can thiazole orange be used to
stain single-stranded DNA? What about its fluorescence strength when
combined with ssDNA?
Thank you!

Ted
Zhejiang University

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  #2  
Old 07-27-2006, 08:45 AM
Duncan Clark
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Default thiazole orange AND ssDNA

Historians believe that in newspost
<[Only registered users see links. ].ne t> on Tue, 25 Jul 2006,
Ted <[Only registered users see links. ]> penned the following literary masterpiece:

Try Pubmed Central

Nucleic Acids Res. 1995 April 11; 23(7): 1215–1222.
Interaction of dimeric intercalating dyes with single-stranded DNA.

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Duncan
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they go flying by.

Duncan Clark
GeneSys Ltd.
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  #3  
Old 07-28-2006, 01:52 AM
Ted
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Default thiazole orange AND ssDNA


I have noted this paper before. But the dye is TOTO in this paper. I think
there maybe some difference between them.
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  #4  
Old 07-28-2006, 08:47 AM
StewJW
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Default thiazole orange AND ssDNA


Why do you want to use thiazole when you can use something like the
established OliGreen? Anyone know the structure of OliGreen, all I can
find out is that its detergent based?

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Can thiazole orange be used to stain single-stranded DNA? shiyan.cn@gmail.com Protocols and Methods Forum 0 07-20-2006 08:47 AM


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