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Fragile X electrophoresis issues

Fragile X electrophoresis issues - Protocols and Methods Forum

Fragile X electrophoresis issues - Post Any Protocol, Method, Technique, Procedure or Tips / Troubleshooting for any Molecular Biology Technique.


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  #1  
Old 07-20-2006, 06:58 PM
lmorgan4523@yahoo.com
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Default Fragile X electrophoresis issues



We have been running Fragile X for about three years now on all the
same equipment. Over the past two months we have seen our southern
gels running very fast. We used to run the (0.8%) agarose gels 50V for
16-18 hours. Due to the increase in rate we have found that we can't
run the gels for longer than 13hours. The display shows that the
voltage is constant at 50V. We have tried new gel boxes, new power
supplies, new buffer, new rooms, new outlets etc etc. Has anyone had
similar issues? Or does anyone have any suggestions to troubleshoot?

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Old 07-21-2006, 08:23 PM
Pow Joshi
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Default Fragile X electrophoresis issues

> We have been running Fragile X for about three years now on all the

seems to me that you've covered all grounds ..... have you changed
your agarose recently? .... and do you see any difference in the
pattern, when you do the southerns?

pow

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