Real time SYBR PCR primer designing

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Hello Debashish,

As the only difference between a normal PCRand a real time PCR is that the PCR products can be detected in real time,meaning that the accumulation of PCR products could be visualized at each cycle using an intercalating dye or a UV source and CCD camera. So As such,there should be no diffrence as far as designing of primers is concerned but as a precautionary step against EtBr interference, which doesn't seem tobe logical anyways, I would advice you to keep the lengh of primer bit shorter then the usual length, that you would have used in normal PCR. Ofcourse, it shouldn't be at the cost of specificity. try to use Primer Designing package in Genomatix.

Many Regards
Tarun Gupta
B.Sc (Hons) Bioinformatics
GGDSD College of Post Graduation,
(Panjab university)
Chandigarh -India

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Mob: 91-9888237906




Tarun Gupta
Summer Trainee
Regional Researchlaboratory (CSIR)
Jammu

B.Sc.(hons)Bioinformatics
GGDSD College of Post Graduation
(Panjab University)
Sector-32/C
Chandigarh-160031
Mob: +91-9888237906

[Only registered users see links. ]
[Only registered users see links. ]