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PCR Self Adjusting Magnesium concentration

PCR Self Adjusting Magnesium concentration - Protocols and Methods Forum

PCR Self Adjusting Magnesium concentration - Post Any Protocol, Method, Technique, Procedure or Tips / Troubleshooting for any Molecular Biology Technique.


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Old 07-06-2006, 02:50 PM
Tim Fitzwater
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Default PCR Self Adjusting Magnesium concentration




Tim Fitzwater wrote: > A quick check of the MSDS for this product reveals that the secret chelator is L-proline at somewhere between 1 and 5% (w/v) for 25 mM magnesium acetate at the 10x buffer concentration. I have not tried it yet. > > Tim Fitzwater
Thanks Tim That's interesting. I didn't see anything about proline in the MSDS - how were you able to work that out? And does anybody know anything about proline and Mg chelation?

The MSDS pdf is available at [Only registered users see links. ] listed at HotMaster Taq DNA polymerase. The 10x concentration is probably 100 mM L-proline, since that is equal to 1.3% (w/v) and falls into the range mentioned in the MSDS of 1 - 5% (w/v). That concentration of proline is for a 10x magnesium acetate concentration of 25 mM. 1x proline at 10 mM and 2.5 mM magnesium seems reasonable
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