I have an urgent question for the forum.
I'm doing KMnO4-footprinting experiments and unfortunately, BME
disturbes the KMnO4 reaction (it is also used to quench the reaction
later). Since there is BME in the buffers of the proteins used in my
reactions, I have to get rid of almost all the BME in the protein
Does anybody now how to do this without damaging the sensitive
Thank you very much.
Dipl.-Biol. Sebastian Grünberg
Lehrstuhl für Mikrobiologie
93053 Regensburg; Germany
Tel.: ++49-941-943 3146
Fax.: ++49-941-943 2403