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How to get rid of DNA in cell lysate

How to get rid of DNA in cell lysate - Protocols and Methods Forum

How to get rid of DNA in cell lysate - Post Any Protocol, Method, Technique, Procedure or Tips / Troubleshooting for any Molecular Biology Technique.


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  #1  
Old 06-26-2006, 12:54 AM
Maryam
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Default How to get rid of DNA in cell lysate



I lysed the fibroblast with 1% triton in PBS by freeze thawing. After loading on the SDS gel I don't see any protein in the fraction. My antibody is specific and it works well with the cell lysate containing PBS alone.

I was wondering if the Presence of DNA in cell lysate mask the appearance of the protein on SDS gel and how to get rid of that.
Or the problem is something else.
Any help will be appreciated.


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Old 06-26-2006, 10:19 PM
DK
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Default How to get rid of DNA in cell lysate

In article <[Only registered users see links. ].net >, Maryam <[Only registered users see links. ]> wrote:

Freeze thawing alone is enough to release >80% of the cytoplasmic
proteins into low speed supernatant even without lysing membranes
with Triton.


Very, very unlikely that it does.


Sonicate the samples if you suspect it is DNA causing your problems.

DK
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