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Problems with QuickChange mutagenesis kit

Problems with QuickChange mutagenesis kit - Protocols and Methods Forum

Problems with QuickChange mutagenesis kit - Post Any Protocol, Method, Technique, Procedure or Tips / Troubleshooting for any Molecular Biology Technique.


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Old 06-09-2006, 02:35 PM
Yulia Shifrin
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Default Problems with QuickChange mutagenesis kit



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Old 06-10-2006, 03:39 AM
DK
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Default Problems with QuickChange mutagenesis kit

In article <[Only registered users see links. ].net >, "Yulia Shifrin" <[Only registered users see links. ]> wrote:

But keep in mind that Stratagene's magic "Quik Solution" supplied with
the kit and recommended to be used at ~ 7% *is* pure DMSO.




The control really should work. Assuming you follow the instructions
properly, some component if your kit is dead. Could be polymerase or
dNTPs but IMHO most likely culprit is the competent cells - which
are not great to begin with. Test them.

DK
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