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Commercial VS column cleaned detergents?

Commercial VS column cleaned detergents? - Protocols and Methods Forum

Commercial VS column cleaned detergents? - Post Any Protocol, Method, Technique, Procedure or Tips / Troubleshooting for any Molecular Biology Technique.


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  #1  
Old 06-08-2006, 07:05 AM
scott.coutts@med.monash.edu.au
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Default Commercial VS column cleaned detergents?



Does anyone know how a commercial protein grade or ultrapure detergent,
such as a Tween, compares to one that I could prepare myself by column
cleaning with activated carbon or amberlite ion-exchange resin? I'd
prefer just to buy it if I could. The problem is, I dont know what
levels of contaminants are in the carbon-cleaned detergents. So
basically I guess I want to know if the commercial protein grade or
ultrapure will be at least as good as carbon-cleaned detergents.

Cheers,

Scott.

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  #2  
Old 06-11-2006, 04:11 PM
Dr Engelbert Buxbaum
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Default Commercial VS column cleaned detergents?

[Only registered users see links. ] wrote:


The issue with some non-ionic detergents is peroxide-formation. These
react with lipids and proteins during solubilisation. For purification
protocolls see for example

Ashani & Catravas Anal. Biochem. 109 (1980) 55-62
Dijkstra et al. Anal. Biochem. 240 (1996) 142-147

The procedures are somewhat involved and use solvents like
dichloromethane, so it might be a good idea to just buy the stuff.

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  #3  
Old 06-13-2006, 12:59 PM
scott.coutts@med.monash.edu.au
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Default Commercial VS column cleaned detergents?


Dr Engelbert Buxbaum wrote:

Hi,

Thanks for the info. But what I really would like to know is how the
level of contaminating peroxides and free fatty acids in activated
carbon-cleaned resins would compare with commercial protein grade or
ultrapure. If I could, I would just do a comparison, but I have no way
of assessing this. (As it happens, I dont actually need it for protein
purification. I only mentioned protein grade detergents because they
are a common grade of purity offered by mol bio companies)

Cheers,

Scott.

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