I am working on plant transformation and I have a plant expression vector
with a novel gene construct. Now I want the blank cultures of Agrobacterium
strains, LBA 4404, EHA 105 and EHA 101 so that I can introduce my vector
plasmid into them and initiate the plant transformation work. Can you please
tell me how to get these Agrobacterium strains.
Consider it as urgent and respond quickly.
Plant transformation Laboratory,
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There are microbiological type culture collection, for example in
Göttingen, Germany. They keep certified strains and make them available
to the scientific community against a fee to cover their costs.
Alternatively, you can ask collegues working with the strains for a
sample, then you loose the advantage of certification.
There is an excellent review by Hellens et al (2000) Trends in Plant
Science that provides contact info for obtaining a number of Agro
strains and binary vectors. I'm on the road right now or I'd provide
a better reference, but it's a good one to have for anybody doing
Although Beth Hood can typically provide the EHA strains, when I last
contacted her, she was having logistically problems in doing so and
directed me to Stan Gelvin at Purdue University.
Although LBA4404 is pretty commonly used, I can't off the top of my
head point you to a specific source. I should think that you should
be able to obtain it from a geographically nearby colleage, however.
Hope this helps.
On Jun 8, 2006, at 7:13 AM, Dr Engelbert Buxbaum wrote: