We work with BL21DE3pLysS and recently most of coltures lysis. We have
just change antibioticos but we have the same resultes.
Did someone have the same problem? And do you have an idea in order to
Last time I saw that problem it turned out that bacterial stocks (or stock
solutions) suffered from a low-level phage contamination. Maxiprep
cultures lysed approximately half-way through their growth.
On Wed, 10 May 2006 14:20:55 +0200, mattia <[Only registered users see links. ]>
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"mattia" <[Only registered users see links. ]> wrote:
I would suspect phage contamination. If it is phage contamination a
phage resistant strain is your best option - decontamination is
practically very difficult. Phage resistant versions of Bl21(DE3) etc.
are available from several vendors.