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subcloning linker problems and need help..please!!

subcloning linker problems and need help..please!! - Protocols and Methods Forum

subcloning linker problems and need help..please!! - Post Any Protocol, Method, Technique, Procedure or Tips / Troubleshooting for any Molecular Biology Technique.


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Old 05-05-2006, 12:05 PM
Debashish Das
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Default subcloning linker problems and need help..please!!




Hi

i would like to thank you all for those tips.

the linker is there in the original as it has been squenced and we can see it. there is no
restriction site within the
linker. as suggested there could be recombination since it is repeatative sequence of Gs and
Cs. we will give a shot
with the RecA- bacts. what i do not understand is that another guy from the lab cloned
another gene into this
pLox and he had the linker still intact. he also used XL10 comps. is it possible that
recombination is kind of "gene
cloned in" sensetive?? that sounds pretty new effect??

best of things
deb

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